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Analisi del DNA e probabilità

Davide Sisti

Abstract


DNA is a long molecule, which is the basis of the chemistry of living beings. The DNA of an individual is the cause of its uniqueness, whether it is a unicellular organism, a plant or an animal. Until about two decades ago the study of DNA was very difficult, but now PCR (polymerase chain reaction) generates in a short time specific sequences of DNA which initially are present only in a small quantity. The possible sources of DNA can be wide-ranging; consider that in simple contact, cells of our skin may separate in flakes containing DNA; from such samples PCR can read sequences of the DNA. Generally DNA analysis allows us to reach a percentage of attribution with an error of about 1/1012 (which means an error every 1000 billion analyses). Nevertheless, the extreme ease of leaving traces of DNA is the Achilles heel of biomolecular methods: if at a crime scene other people have passed, if the victim had contact with someone else before his death, if the objects have been touched by others in the past, different patterns of DNA will be found, which must, of course, be contextualized in order not to make serious errors of interpretation.

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References


Doyle, A.C. (1892), “A Case Of Identity”, in The Penguin Complete Sherlock Holmes, London, 1981: 190-201.

Guescini, M., D. Sisti, M.B. Rocchi, L. Stocchi, V. Stocchi (2008), “A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition”, BMC bioinformatics, 9: 326.

[Guescini, M., D. Sisti, R. Panebianco], “Cy0: A new approach in PCR quantification”, http://www.cy0method.org/index.php.

Mullis, K. (1998), Dancing Naked in the Mind Field, trad. it. Ballando nudi nel campo della mente, Milano, Baldini & Castoldi, 2000.

Piattelli Palmarini, M. (2004), “«Cosi creai la fotocopiatrice del Dna» – Intervista allo scienziato Usa Kary Mullis”, Corriere della Sera, 6 ottobre 2004, 19.






 


Linguæ & - Rivista di lingue e culture moderne
Registered by Tribunale di Milano (06/04/2012 n. 185)
Online ISSN 1724-8698 - Print ISSN 2281-8952


Dipartimento di Scienze della Comunicazione, Studi Umanistici e Internazionali: Storia, Culture, Lingue, Letterature, Arti, Media
Università degli Studi di Urbino Carlo Bo


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